Laboratory of Systems Virology

                              

   Director  -  Andrey V. Vasin, PhD

   Lab. telephone: +7 (812) 499–15–24

   E-mail:  andrey.vasin@influenza.spb.ru

The Laboratory of Systemic Virology was founded in 2016 on the basis of the Laboratory of Structural and Functional Proteomics.

Main activities: 

  • Search for viral and cellular targets useful for antiviral drug development
  • Study of the mechanisms behind current and potential antiviral drugs using modern methods such as surface plasmon resonance, MALDI mass spectrometry, next generation sequencing, single and two-dimensional electrophoretic separation of proteins, and atomic force microscopy
  • Characterization of the composition and structural components of experimental vaccine batches being developed at the Smorodintsev Research Institute of Influenza (Russian Ministry of Health)
  • Determination of the binding parameters of diagnostic monoclonal antibodies with antigens using surface plasmon resonance
  • Development of new methodological approaches for the diagnosis of inflammatory processes in the body, including sepsis, based on mass spectrometry
  • Study of the fundamentals of the interaction between the influenza virus and cells by proteomic and next generation sequencing methods

Achievements

As part of the federal targeted program “Development of the Russian pharmaceutical and medical sector until 2020 and beyond,” a new peptide-based antiviral preparation is being developed and tested, including preclinical studies together with other Smorodintsev Research Institute of Influenza laboratories (Matusevich O.V. et al., 2014; Egorov et al., International Journal of Peptides, 2013).

Projects

“Preclinical studies of an antiviral, peptide-based drug that suppresses the replication of human influenza A (H1N1) viruses” (state contract 14.N08.11.0080, federal targeted program “Development of the Russian pharmaceutical and medical sector until 2020 and beyond”)

"Study of the physicochemical and immunogenic properties of virus-like particles based on the core antigen of the hepatitis B virus (HBc) and the ectodomain of the M2 protein of influenza A virus" (state project, 2016)

International and inter-institutional scientific collaborations

The Laboratory of Systemic Virology collaborates with the Kurchatov Institute National Research Center (Russia), the I.M. Franca Laboratory of Neutron Physics (Joint Institute for Nuclear Research, Dubna, Russia), Jülich Center for Neutron Science (Munich, Germany), and the European Synchrotron Radiation Facility (Grenoble, France) on structural studies of viral and cellular proteins.

Selected publications

  1. Egorov V.V., Lebedev D.V., Isaev-Ivanov V.V., Gorshkov A.N., Vasin A.V., Kiselev O.I., Murugova T.N. CHARACTERIZATION OF OLIGOMERIZATION OF A PEPTIDE FROM THE EBOLA VIRUS GLYCOPROTEIN BY SMALL-ANGLE NEUTRON SCATTERING Crystallography Reports. 2016. . 61. № 1. . 94-97.
  2. Kadochnikov V.V., Egorov V.V., Shvetsov A.V., Isaev-Ivanov V.V., Lebedev D.V., Kuklin A.I. MODELING OF CONFORMATIONAL TRANSITIONS OF FIBRILLOGENIC PEPTIDE, HOMOLOGOUS TO BETA-DOMAIN OF HUMAN ALPHA-LACTALBUMIN Crystallography Reports. 2016. . 61. № 1. . 98-105.
  3. Sivak K.V., Vasin A.V., Egorov V.V., Tsevtkov V.B., Kuzmich N.N., Savina V.A., Kiselev O.I. ADENOSINE A2A RECEPTOR AS A DRUG TARGET FOR TREATMENT OF SEPSIS Molecular Biology. 2016. . 50. № 2. . 200-212.
  4. Egorov V.V., Lebedev D.V., Shvetsov A.V., Isaev-Ivanov V.V., Shaldzhyan A.A., Gorshkov A.N., Zabrodskaya Ya.A., Ksenofontova O.I., Tsybalova L.M., Kuklin A.I., Vasin A.V. ON THE STRUCTURAL FEATURES OF INFLUENZA A NUCLEOPROTEIN PARTICLES FROM SMALL-ANGLE X-RAY SCATTERING DATA Journal of Surface Investigation: X-Ray, Synchrotron and Neutron Techniques. 2016. . 10. № 2. . 322-325.
  5. Heydarov R.N., Fesenko E.E., Shaskolskiy B.L., Titov S.V., Dementieva E.I., Zasedatelev A.S., Mikhailovich V.M., Klotchenko S.A., Vasin A.V., Kiselev O.I. IDENTIFICATION OF GENETIC DETERMINANTS OF INFLUENZA A VIRUS RESISTANCE TO ADAMANTANES AND NEURAMINIDASE INHIBITORS USING BIOLOGICAL MICROARRAY Doklady Biochemistry and Biophysics. 2015. . 460. № 1. . 4-8.
  6. Vasin A.V., Petrova A.V., Egorov V.V., Plotnikova M.A., Klotchenko S.A., Kiselev O.I., Karpenko M.N. THE INFLUENZA A VIRUS NS GENOME SEGMENT DISPLAYS LINEAGE-SPECIFIC PATTERNS IN PREDICTED RNA SECONDARY STRUCTURE BMC Research Notes. 2016. . 9. № 1. . 279.
  7. Rusinov V.L., Sapozhnikova I.M., Ulomskii E.N., Medvedeva N.R., Chupakhin O.N., Egorov V.V., Kiselev O.I., Deeva E.G., Vasin A.V. NUCLEOPHILIC SUBSTITUTION OF NITRO GROUP IN NITROTRIAZOLOTRIAZINES AS A MODEL OF POTENTIAL INTERACTION WITH CYSTEINE-CONTAINING PROTEINS Chemistry of Heterocyclic Compounds. 2015. . 51. № 3. . 275-280.
  8. Kiselev O.I., Vasin A.V., Deeva E.G., Sivak K.V., Egorov V.V., Tsvetkov V.B., Egorov A.Y., Romanovskaya-Romanko E.A., Stepanova L.A., Komissarov A.B., Tsybalova L.M., Ignatjev G.M., Shevyryova M.P. EBOLA HEMORRHAGIC FEVER: PROPERTIES OF THE PATHOGEN AND DEVELOPMENT OF VACCINES AND CHEMOTHERAPEUTIC AGENTS Molecular Biology. 2015. . 49. № 4. . 480-493.
  9. Egorov V., Vasin A., Lebedev D., Grudinina N. PEPTIDE-INDUCED AMYLOID-LIKE CONFORMATIONAL TRANSITIONS IN PROTEINS International Journal of Peptides. 2015. . 2015. . 723186.
  10. Prochukhanova A.R., Azarenok A.A., Nazarova A.V., Zhilinskaya I.N., Lublinskaya O.G., Zenin V.V. ALTERATIONS IN CELL CYCLE DYNAMICS IN HUMAN ENDOTHELIUM CELL CULTURE INFECTED WITH INFLUENZA VIRUS Cell and Tissue Biology. 2015. . 9. № 4. . 326-329.
  11. Matusevich O.V., Gluzdikov I.A., Titov M.I., Egorov V.V., Zarubaev V.V., Shtro A.A., Slita A.V., Dukov M.I., Shurygina A.P.S., Smirnova T.D., Vasin A.V., Kiselev O.I., Kudryavtsev I.V. SYNTHESIS AND ANTIVIRAL ACTIVITY OF PB1 COMPONENT OF THE INFLUENZA A RNA POLYMERASE PEPTIDE FRAGMENTS Antiviral Research. 2014. . 113. . 4-10.
  12. Vasin A.V., Temkina O.A., Egorov V.V., Klotchenko S.A., Plotnikova M.A., Kiselev O.I. MOLECULAR MECHANISMS ENHANCING THE PROTEOME OF INFLUENZA A VIRUSES: AN OVERVIEW OF RECENTLY DISCOVERED PROTEINS Virus Research. 2014. . 185. . 53-63.
  13. Ksenofontova O.I., Vasin A.V., Egorov V.V., Kiselev O.I., Bobyl A.V., Soldatenkov F.Y., Terukov E.I., Ulin V.P., Ulin N.V. POROUS SILICON AND ITS APPLICATIONS IN BIOLOGY AND MEDICINE Technical Physics. The Russian Journal of Applied Physics. 2014. . 59. № 1. . 66-77.
  14. Sultankulova K.T., Chervyakova O.V., Kozhabergenov N.S., Shorayeva K.A., Strochkov V.M., Orynbayev M.B., Sandybayev N.T., Sansyzbay A.R., Vasin A.V. COMPARATIVE EVALUATION OF EFFECTIVENESS OF IAVCHIP DNA MICROARRAY IN INFLUENZA A DIAGNOSIS TheScientificWorldJournal [electronic resource]. 2014. . 2014. . 620580.
  15. Egorov V.V., Shaldzhyan A.A., Sirotkin A.K., Gorshkov A.N., Mirgorodskaya O.A., Vasin A.V., Lebedev D.V., Grudinina N.A., Shavlovsky M.M. A CONSERVATIVE MUTANT OF A PROTEOLYTIC FRAGMENT PRODUCED DURING FIBRIL FORMATION ENHANCES FIBRILLOGENESIS Prion. 2014. . 8. № 5. . 369-373.
  16. Egorov V.V., Zabrodskaya Ya.A., Vasin A.V., Klotchenko S.A., Lebedev D.V., Kalinin A.S., Kolikov V.A.,Grudinina N.A., Shawlovsky M.M., Rutberg P.G. MAGNETIC LABELING OF PROTEINS FOR ATOMICFORCE MICROSCOPY Doklady Biochemistry and Biophysics. 2013. . 448. № 1. . 33-35.
  17. Egorov V.V., Shaldzhyan A.A., Slita A.V., Sirotkin A.K., Vasin A.V., Grudinina N.A., Shavlovsky M.M.,Lebedev D.V. AMYLOIDOGENIC PEPTIDE HOMOLOGOUS TO FRAGMENT 129-148 OF HUMANMYOCILIN Prion. 2013. . 7. № 3. . 248-252.
  18. Vasin A., Klotchenko S., Puchkova L. PHYLOGENETIC ANALYSIS OF SIX-DOMAIN MULTI-COPPER BLUE PROTEINS PLoS Currents. 2013. № MARCH 2013. . A265.
  19. Egorov V.V., Matusevich O.V., Shaldzhyan A.A., Skvortsov A.N., Zabrodskaya Ya.A., Garmay Y.P., Zarubayev V.V., Sirotkin A.K., Vasin A.V., Kiselev O.I., Landa S.B., Lebedev D.V. STRUCTURAL FEATURES OF THE PEPTIDE HOMOLOGOUS TO 6-25 FRAGMENT OF INFLUENZA A PB1 PROTEIN International Journal of Peptides. 2013. . 2013. . 370832.